| Termijn | Thuis | Uitteam | Uitslag |
|---|---|---|---|
| 19-9-25 19:00 | Frosinone Calcio | FC Südtirol | 2:2 |
| 19-9-25 20:45 | US Lecce | Cagliari Calcio | 1:2 |
| 19-9-25 21:00 | Palermo FC | SSC Bari | 2:0 |
| 20-9-25 15:00 | FC Bologna | Genua CFC | 2:1 |
| 20-9-25 15:00 | Spezia Calcio | Juve Stabia | 1:3 |
| 20-9-25 15:00 | Venezia FC | FC Cesena | 1:2 |
| 20-9-25 15:00 | AC Reggiana 1919 | US Catanzaro 1929 | 2:2 |
| 20-9-25 17:15 | AC Monza | Sampdoria Genua | 1:0 |
| 20-9-25 18:00 | Hellas Verona | Juventus Turin | 1:1 |
| 20-9-25 19:30 | Mantova 1911 | Modena FC | 1:3 |
| 20-9-25 20:45 | Udinese Calcio | AC Mailand | 0:3 |
| 21-9-25 12:30 | Lazio Rom | AS Rom | 0:1 |
| 21-9-25 15:00 | US Cremonese | Parma Calcio 1913 | 0:0 |
| 21-9-25 15:00 | FC Turin | Atalanta Bergamo | 0:3 |
| 21-9-25 15:00 | Carrarese Calcio 1908 | US Avellino | 3:4 |
| 21-9-25 17:15 | Pescara Calcio | FC Empoli | 4:0 |
| 21-9-25 18:00 | AC Florenz | Como Calcio | 1:2 |
| 21-9-25 19:30 | Padova Calcio | Virtus Entella | 2:1 |
| 21-9-25 20:45 | Inter Mailand | Sassuolo Calcio | 2:1 |
| 22-9-25 20:45 | SSC Neapel | Pisa Sporting Club | 3:2 |