| Termijn | Thuis | Uitteam | Uitslag |
|---|---|---|---|
| 26-9-25 20:30 | US Catanzaro 1929 | Juve Stabia | 2:2 |
| 27-9-25 15:00 | Como Calcio | US Cremonese | 1:1 |
| 27-9-25 15:00 | US Avellino | Virtus Entella | 2:0 |
| 27-9-25 15:00 | Mantova 1911 | Frosinone Calcio | 1:5 |
| 27-9-25 15:00 | FC Cesena | Palermo FC | 1:1 |
| 27-9-25 15:00 | FC Südtirol | AC Reggiana 1919 | 3:1 |
| 27-9-25 15:00 | Venezia FC | Spezia Calcio | 2:0 |
| 27-9-25 17:15 | AC Monza | Padova Calcio | 0:1 |
| 27-9-25 18:00 | Juventus Turin | Atalanta Bergamo | 1:1 |
| 27-9-25 19:30 | SSC Bari | Sampdoria Genua | 1:1 |
| 27-9-25 20:45 | Cagliari Calcio | Inter Mailand | 0:2 |
| 28-9-25 12:30 | Sassuolo Calcio | Udinese Calcio | 3:1 |
| 28-9-25 15:00 | Pisa Sporting Club | AC Florenz | 0:0 |
| 28-9-25 15:00 | AS Rom | Hellas Verona | 2:0 |
| 28-9-25 17:15 | Modena FC | Pescara Calcio | 2:1 |
| 28-9-25 18:00 | US Lecce | FC Bologna | 2:2 |
| 28-9-25 19:30 | FC Empoli | Carrarese Calcio 1908 | 2:2 |
| 28-9-25 20:45 | AC Mailand | SSC Neapel | 2:1 |
| 29-9-25 18:30 | Parma Calcio 1913 | FC Turin | 2:1 |
| 29-9-25 20:45 | Genua CFC | Lazio Rom | 0:3 |